Coding
CLuc

Part:BBa_K2845002:Design

Designed by: Antonio Mart�nez, Jan Kr�semann, Eline Bijman   Group: iGEM18_ETH_Zurich   (2018-10-10)


C-terminus of split EPIC luciferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 419
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 123


Design Notes

Coding region for the C-terminus of split EPIC Luciferase (BBa_K325101) (Luker et al.)

Sources

Luker, Kathryn E., et al. “Kinetics of Regulated Protein – Protein Interactions Revealed with Firefly Luciferase Complementation Imaging in Cells and Living Animals.” PNAS, vol. 101, no. 33, 2004.